Stabilization of Blood Homocysteine by 3-Deazaadenosine
نویسندگان
چکیده
منابع مشابه
Effects of temperature on stability of blood homocysteine in collection tubes containing 3-deazaadenosine.
BACKGROUND The accuracy of homocysteine (Hcy) results is currently compromised by the requirement to separate the plasma within 1 h of sample collection. We studied the effect of temperature on the stability of plasma Hcy over a 72-h time course in blood collected into evacuated tubes containing either EDTA alone or both EDTA and 3-deazaadenosine (3DA). METHODS We recruited 100 volunteers, in...
متن کامل3-Deazaadenosine, a Stabilizer of Whole-Blood Homocysteine Content, Does Not Interfere with the Single- Enzyme Homocysteine Assay while Totally Inhibiting the Enzyme Conversion Homocysteine Immunoassay
eclampsia (at 34 weeks of gestation) had sFlt1 values within the reference interval. This woman had abruptio placenta at delivery (36 weeks of gestation). In the remaining seven patients, the sFlt1 assay would have predicted preeclampsia a mean of 11.2 weeks (range, 6.5–16.5 weeks) before clinical onset. No significant difference was observed in mean (SD) serum creatinine concentrations ( mol/L...
متن کاملAddition of 3-deazaadenosine to vacutainer tubes stabilizes whole-blood homocysteine for at least 6 hours at ambient temperature.
To the Editor: Increased plasma homocysteine is now well established as a risk factor for cardiac, cerebral, and peripheral vascular disease (1). Despite this, the test is not widely available. This is partly because of practical difficulties in sample collection. The homocys-teine concentration increases in whole blood by up to 10% per hour unless samples are kept on ice and separated within 6...
متن کاملInhibition of murine erythroleukemia cell differentiation by 3-deazaadenosine.
Recent studies have demonstrated that 5'-methylthioadenosine, an inhibitor of S-adenosylhomocysteine (AdoHcy) hydrolase, blocks induction of murine erythroleukemia cell (MEL) differentiation. The nucleoside analogue 3-deazaadenosine (c3Ado) is both an efficient substrate and a potent inhibitor of AdoHcy hydrolase. The present study was undertaken to determine whether c3Ado would similarly inhib...
متن کاملPharmacokinetics of the antiviral agent carbocyclic 3-deazaadenosine.
The pharmacokinetics of carbocyclic 3-deazaadenosine (Cc3Ado), a competitive inhibitor of S-adenosyl-L-homocysteine hydrolase and novel antiviral agent, was investigated in female BALB/c mice. Mice were dosed either orally or intravenously with a single dose of 10 mg/kg (10 microCi [3H]Cc3Ado), and blood and tissue samples were taken at selected intervals for 24 hr. The plasma concentration vs....
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ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 1998
ISSN: 0004-5632,1758-1001
DOI: 10.1177/000456329803500612